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ASTM F2997 PDF
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Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis
| Published by | Publication Date | Number of Pages |
| ASTM | 06/15/2021 | 17 |
ASTM F2997 – Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture ofProgenitor Cells Using Fluorescent Image Analysis
1.1”This practice defines a method for the estimation of calcium content at multiple time points in living cell cultures that have been cultured under conditions known to promote mineralization. The practice involves applying a fluorescent calcium-chelating dye that binds to the calcium phosphate mineral crystals present in the live cultures followed by image analysis of fluorescence microscopy images of the stained cell cultures. Quantification of the positively stained areas provides a relative measure of the calcium content in the cell culture plate. A precise correlation between the image analysis parameters and calcium content is beyond the scope of this practice.
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