ASTM E3354 PDF

1.1 This guide provides a framework for the application of molecular biological tools (MBTs) to assess and characterize in-situ biological processes to improve contaminated soil and groundwater management. While the focus of this guide is on in-situ biological processes, some concepts of how to apply MBTs can also be applied to ex-situ bioremediation approaches (for example, biopiles, bioreactors) to support design, operation, and troubleshooting. The intent of this guide is to develop a consistent way in which MBTs are applied at contaminated sites, not to develop expertise. Technical experts need to be engaged whenscoping, planning, executing, and interpreting data for MBTs. Lastly, there is a brief description of isotopic techniques within section 5.2; however, the scope and focus of this guide is the use of nucleic acid-based MBTs to assess biological processes at contaminated sites.