ASTM E3273 PDF

1.1”This practice is to assess technologies for microbial decontamination of indoor air using a sealed, room-sized chamber (~24”’m³) as recommended by the U.S. Environmental Protection Agency (3). The test microbe is aerosolized inside the chamber where a fan uniformly mixes the aerosols and keeps them airborne. Samples of the air are collected and assayed, firstly to determine the rates of physical and biological decay of the test microbe, and then to assess the air decontaminating activity of the technology under test as log₁₀ or percentage reductions in viability per m³ (1). The air temperature and relative humidity (RH) in the chamber are measured and recorded during each test.